This article examines the impact of adequate regional anesthesia achieved through ultrasound-guided ESP blockade on the quality of diagnostic specimen collection during multifocal prostate biopsy MFB . A comparative analysis was conducted between the ESP blockade group N=53 and the standard local anesthesia group N=55 . Biopsy specimen quality was assessed based on core length and fragmentation, which correlated with diagnostic yield PCa detection . The findings suggest that patient comfort, owing to ESP blockade, results in higher-quality and less fragmented specimens, potentially increasing the overall sensitivity of MFB.